Prime Minister Scott Morrison has vowed to crack down on GetUp, accusing the activist group of bullying, misogyny and undisclosed links to the Labor Party.
Mr Morrison hinted at another inquiry into the independence of the organisation, which he said was "no longer a wolf in sheep's clothing".
"GetUp is a wolf in wolf's clothing and I think Australians are sick of that," he told reporters at the South Australian Liberal state council on Saturday.
Liberal MP Nicolle Flint said the government should consider a move to register GetUp as a political party to ensure accountability and transparency.
Ms Flint has accused the group of waging a campaign to personally "destroy" her in the lead up to the election, by shouting at her, defacing her posters and egging her office.
"They are a front for Labor and the Greens," she said.
GetUp national director Paul Oosting hit back at Scott Morrison's comments as undemocratic and baseless.
"The prime minister is levelling extraordinary attacks on everyday people who participated in politics this election - with no evidence," he said.
Mr Oosting said a request that the Australian Electoral Commission investigate GetUp's independence would be a "political stitch-up" after the commission ruled in the group's favour in February.
"This would be the fourth attempt by the hard right to shut down independent grassroots campaigning," he said.
Mr Morrison told delegates and observers at Saturday's meeting that GetUp works on the principle of "plausible deniability".
"They say they aren't Labor when we all know they are," he said.
"They deny having anything to do with the vile, personal attacks on our candidates that only occur in seats where Getup are running campaigns."
He said the organisation should be ashamed of what happened in Nicole Flint's seat of Boothby, as it was "misogyny" and "bullying".
Labor spokesman Mark Butler slammed the idea that GetUp be subject to the same scrutiny as political parties as unnecessary.
"They're clearly not a political party, they're not running candidates," he said.
"I think there are strong programs and strong arrangements in place to make sure that any third party that wants to participate in Australia's democracy... is subject to appropriate regulation and transparency.
"This looks like an obsession by the hard right within the coalition party room."
Australian Associated Press